Description

Introduction

Helicobacter pylori (H. pylori) is a type of bacteria that can reside in the stomach lining, causing many dangerous digestive diseases, including:

Peptic ulcer disease: H. pylori is the main cause of peptic ulcer disease, leading to symptoms such as heartburn, indigestion, abdominal pain, nausea and vomiting.
Stomach cancer: H. pylori infection is a high risk factor for stomach cancer, one of the most common cancers in the world.
Chronic gastritis: H. pylori can cause chronic gastritis, leading to symptoms such as abdominal pain, bloating, indigestion and diarrhea.

AccuPid H. pylori Genotyping Kit uses real-time PCR (qPCR) technique that allows identification of the bacterial-specific cagA, vacA s1/s2 and m1/m2 gene regions of H. pylori in samples quickly and accurately. High accuracy and sensitivity, while minimizing the risk of foreign contamination compared to other PCR techniques.

Benefits

Accurate diagnosis: Accurately detect the H. pylori strain that causes the disease, helping to direct effective treatment.
Appropriate treatment regimen: Determine the drug resistance of H. pylori, help choose an appropriate treatment regimen, and limit the risk of relapse.
Reduce the risk of complications: Early diagnosis and effective treatment of H. pylori helps reduce the risk of dangerous complications such as stomach cancer.
Effective health management: Provides important information to effectively monitor and manage health after H. pylori treatment.

Principles

The AccuLite H. pylori strain typing test kit (RUO) uses real-time PCR (PCR) technology to amplify and detect characteristic DNA sequences associated with different H. pylori strains. This highly sensitive technique targets the vacA and cagA genes, which play important roles in bacterial virulence and drug resistance mechanisms.

Testing procedure

Sample collection: Gastric biopsy samples are taken from the patient’s gastric mucosa during endoscopy.

DNA extraction: DNA is extracted from the biopsy specimen using specialized methods to ensure purity and integrity.

Real-time PCR amplification: The extracted DNA was amplified by real-time PCR using primers specific for the vacA and cagA genes.

Fragment analysis: Amplified DNA fragments are analyzed based on their size and fluorescence signal intensity, allowing identification of the H. pylori strain.

References

1. https://wwwnc.cdc.gov/travel/yellowbook/2024/infections-diseases/helicobacter pylori#:~:text=Although%20usually%20asymptomatic%2C%20H.,the%20World%20Health%20Organization%2C%20H.